Enzyme + Bacteria
The aim of adding enzymes to silage is usually to aid the breakdown of plant cell walls (e.g. use of celluloses and hemi-celluloses). The main benefit of this appears to be an increase in the amount of sugars available for LAB bacteria to convert to lactic acid for more rapid acidification.
While there is some evidence of favourable outcomes of this on silage quality and animal production, there is very little evidence suggesting that the enzymes will have a positive impact in aiding the Bio-methane yield.
An additive with enzyme is not cost effective and the effect of the enzymes is negligible due to the abundance of free sugars in silage to create good fermentation. Biogas plants have a typically good retention time, therefore the “supposed” benefit of increasing digestibility in the AD is not realistic as the quantity of enzyme required would need to be at a much higher concentration.
The below independent study demonstrates that at 49 days with aerobic stress, (typical on site AD clamp) an additive that combines bacteria and enzymes, LAB-he A (Lactobacillus buchneri + Apergillus oryzae β-glucanase and α-amylase, Trichoderma longibrachiatum xylanase) was unable to produce significant positive results in bio-methane production and reduce dry matter losses when compared to biological additives with homo and hetero fermentative lactic acid bacteria.
“…LAB-ho+he B (Silasil Energy) were effective to completely avoid aerobic deterioration during 7 days exposure to air…”
“…Highest methane yields were analysed with addition of LAB-ho+he B. Under air stress conditions, treatment of maize with the inoculant LAB-ho+he B significantly increased the methane yield…”
Dr Pat Hoffman and Dr Richard Muck of the USDA Forage Research Centre have stated, “Enzymes can improve silage fermentation when the substrate (e.g., sugars) is limiting. Soluble sugars are required to help bacteria produce lactic acid, which is required to lower silage pH for proper fermentation. Generally, enzyme addition to silages has a small effect on fermentation.”
If a product claims to contain enzymes, the label should clearly state guaranteed levels. It’s not enough to simply list some enzyme sources in the ingredients. Without guaranteed levels, you can assume that any enzyme activity present is limited at best. To be effective, a product must contain guaranteed levels that are validated effective by research studies.